this post was submitted on 18 Sep 2023
13 points (93.3% liked)

mycology

403 readers
1 users here now

Mycology

be careful about self-doxxing

founded 2 years ago
MODERATORS
 

Plz help me mushroom mods i suck at this help help help i spill my jice

top 6 comments
sorted by: hot top controversial new old
[–] [email protected] 4 points 2 years ago (1 children)

Like is it in a reusable container? You could do a spore print of one of the mature mushrooms?

[–] [email protected] 4 points 2 years ago (1 children)

Would you get a spore print from an oyster? I have very limited experience in culinary mushrooms, but when I did oysters, I don't remember them dropping spores

[–] [email protected] 6 points 2 years ago (1 children)

There is a sporeless oyster species, but most of them do drop spores that can be collected on a piece of tin foil by letting it sit on the foil for a few hours. Oyster spores are actually super toxic and six months of exposure without a respirator can cause a lifelong allergy to them, mushroom worker's lung.

[–] [email protected] 4 points 2 years ago

Very interesting! I would assume the all in one kits that people have are the sporeless kind then

[–] [email protected] 4 points 2 years ago

Look up broke boi tek and make the jars as per the recipe. You should be able to take a small chunk of the mycelium and put it in a (sterile) jar from the tek. It should propagate.

[–] [email protected] 3 points 2 years ago* (last edited 2 years ago)

If you haven't done this before, there will be a learning curve especially with regards to aseptic technique. Everything will need to be sterilized properly or you will wind up with contamination and the process will fail.

Usually you can take a chunk of the existing spawn and put it in a new spawn bag to start the process all over again. Results will vary greatly depending on how dried out the spawn is and how dirty (with other microbes) it is. You can also use tissue from the inside of the mushroom (typically from the stipe but oysters don't have much of a stipe) on malt extract agar to grow mycelium on plates. This can then be chopped up and put into spawning bags, jars, or just more plates. Some people make a liquid culture out of the mycelium but agar seemed easier to me so that's what I do. Spore prints are "easier" in the sense that you are less likely to get contamination than with a mycelium culture, but they will generally take longer and there's a chance you wind up with a strain that doesn't fruit as well.